i NOS; Nitric Oxide Synthase, Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN.
Specific References (47) | bs-0162R has been referenced in 47 publications.
111 [IF=2.35] Li, Guofu, et al. "The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats." International Journal of Molecular Medicine 38.3 (2016): 767-775. WB ; Rat. 222
111 [IF=4.56] Shanshan et al. Phospholipase A2 of Peroxiredoxin 6 Plays a Critical Role in Cerebral Ischemia/Reperfusion Inflammatory Injury. (2017) Front.Cell.Neurosc. 11:99 WB ; Rat. 222
111 [IF=2.24] Zhang et al. High altitude increases the expression of hypoxia-inducible factor 1α and inducible nitric oxide synthase with intest-inal mucosal barrier failure in rats. (2015) Int.J.Clin.Exp.Patho. 8:5189-95 IHC ; Rat. 222
111 [IF=5.64] Wang et al. CXCL1 derived from tumor-associated macrophages promotes breast cancer metastasis via activating NF-κB/SOX4 signaling. (2018) Cell.Death.Dis. 9:880 WB ; Mouse. 222
111 [IF=2.8] Liu J et al. Alterations of antioxidant indexes and inflammatory cytokine expression aggravated hepatocellular apoptosis through mitochondrial and death receptor-dependent pathways in Gallus gallus exposed to arsenic and copper. Environ Sci Pollut Res Int WB ; Chicken. 222
111 [IF=2.332] Zhao X et al. Total flavones of fermentation broth by co-culture of Coprinus comatus and Morchella esculenta induces an anti-inflammatory effect on LPS-stimulated RAW264.7 macrophages cells via the MAPK signaling pathway.(2018) Microb Pathog.125:431-437. WB ; Mouse. 222
111 [IF=2.655] Horváth E et al. Ischemic damage and early inflammatory infiltration are different in the core and penumbra lesions of rat brain after transient focal cerebral ischemia. J Neuroimmunol. 2018 Nov 15;324:35-42. IHC-P ; Rat. 222
111 [IF=4.183] Imoto K et al. Periostin Mediates Right Ventricular Failure through Induction of Inducible Nitric Oxide SynthaseExpression in Right Ventricular Fibroblasts from Monocrotaline-Induced Pulmonary Arterial Hypertensive Rats. Int J Mol Sci. 2018 Dec 24;20(1). IHC-P ; Rat. 222
111 [IF=3.118] Lv R et al. Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway. Int Immunopharmacol. 2019 Feb 18;70:28-36. IHC-P ; Rat. 222
111 [IF=2.914] Guo MH et al. Elemental imbalance elicited by arsenic and copper exposure lead to oxidative stress and immunotoxicity in chicken gizzard, activating the protective effects of heat shock proteins. Environmental Science and Pollution Research. 2019. WB ; Chicken. 222
111 [IF=3.87] Jiao F et al. AGK2 Alleviates Lipopolysaccharide Induced Neuroinflammation through Regulation of Mitogen-Activated Protein Kinase Phosphatase-1. J Neuroimmune Pharmacol. 2019 Nov 30. IHF ; Mouse. 222
111 [IF=3.483] Huang P et al. Theoretical basis of nitrosomyoglobin formation in a dry sausage model by coagulase-negative staphylococci: Behavior and expression of nitric oxide synthase. Meat Sci. 2019 Nov 27;161:108022. WB ; coagulase-negative staphylococci (CNS) and P. pentosaceus. 222
111 [IF=3.58] El-Lakany MA et al. Ovariectomy provokes inflammatory and cardiovascular effects of endotoxemia in rats: Dissimilar benefits of hormonal supplements. Toxicol Appl Pharmacol. 2020 Feb 21;393:114928. IHC-P ; Rat. 222
111 [IF=3.361] Wang M et al. Berberine combined with cyclosporine A alleviates acute graft-versus-host disease in murine models. Int Immunopharmacol. 2020 Feb 9;81:106205. IHC-P ; Mouse. 222
111 [IF=3.761] Toru Nishikawa et al. Therapeutic potential for insulin on type 1 diabetes‐associated periodontitis: analysis of experimental periodontitis in streptozotocin‐induced diabetic rats. J Diabetes Investig . 2020 Apr 17. IHC ; rat. 222
111 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673 IHC/IF ; Rat/Mouse. 222
111 [IF=2.5] Yang F et al. The effects of binge alcohol exposure on tooth movement and associated root resorption in rats. Alcohol . 2020 Nov;88:1-9. IHC-P ; Rat. 222
111 [IF=7.919] Chen Y et al. A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activityClin Transl Med.2020 Dec;10(8):e240. WB、IHC ; Mouse. 222
111 [IF=7.727] Xue Wang. et al. Engineered liposomes targeting the gut–CNS Axis for comprehensive therapy of spinal cord injury. J Control Release. 2021 Mar;331:390 WB,IHC ; Mouse. 222
111 [IF=3.909] Qian Xueshen. et al. Periodontitis Deteriorates Cognitive Function and Impairs Neurons and Glia in a Mouse Model of Alzheimer’s Disease. J Alzheimers Dis. 2021 Jan;79(4):1785-1800 WB ; Mouse. 222
111 [IF=3.69] Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919 WB ; Mouse. 222
111 [IF=8.758] Yinying Sheng. et al. Development and In Vitro Biodegradation of Biomimetic Zwitterionic Phosphorylcholine Chitosan Coating on Zn1Mg Alloy. Acs Appl Mater Inter. 2020;12(49):54445–54458 IF ; Mouse. 222
111 [IF=3.368] Liu Tang. et al. Topical Application with Conjugated Linoleic Acid Ameliorates 2, 4‐dinitrofluorobenzene‐Induced Atopic Dermatitis‐like Lesions in BALB/c Mice. Exp Dermatol. 2021 Feb;30(2):237-248 WB ; Mouse. 222
111 [IF=2.965] Sawada Noritaka. et al. Glucagon-Like Peptide-1 Receptor Agonist Liraglutide Ameliorates the Development of Periodontitis. J Diabetes Res. 2020;2020:8843310 IHC ; Rat. 222
111 [IF=5.88] Shuai Chen. et al. Chondroitin sulfate modified 3D porous electrospun nanofiber scaffolds promote cartilage regeneration. Mat Sci Eng C-Mater. 2021 Jan;118:111312 IHC ; Rabbit. 222
111 [IF=8.724] Penghui Zhang. et al. Effect of cyclic mechanical loading on immunoinflammatory microenvironment in biofabricating hydroxyapatite scaffold for bone regeneration. Bioact Mater. 2021 Oct;6:3097 WB ; Mouse. 222
111 [IF=3.943] Kai Kang. et al. FGF21 alleviates chronic inflammatory injury in the aging process through modulating polarization of macrophages. Int Immunopharmacol. 2021 Jul;96:107634 WB ; Mouse. 222
WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 ICC=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量
130kDa
細胞定位
細胞核 細胞漿
性 狀
Liquid
濃 度
1mg/ml
免 疫 原
KLH conjugated synthetic peptide derived from human NOS-2: 1051-1144/1144
亞 型
IgG
純化方法
affinity purified by Protein A
緩 沖 液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).
Function:
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.
Subunit:
Homodimer. Binds SLC9A3R1.
Tissue Specificity:
Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
Similarity:
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain.
合成與降解（Synthesis and Degradation）
催化生物體內一氧化氮（NO）生成的酶。分神經型一氧化氮合成的酶（nNOS or NOS-1）、誘導型一氧化氮合成的酶（iNOS or NOS-2）、內皮型一氧化氮合成的酶（eNOS or NOS-3）。
產品圖片
Sample:U251 Cell Lysate at 40 ug
Primary: Anti-iNOS(bs-0162R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 130kD
Observed band size: 130kD
Sample:
Lane 1: Mouse Raw264.7 tissue lysates
Lane 2: Human Jurkat cell lysates
Primary: Anti-iNOS (bs-0162R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kDa
Observed band size: 140 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (inos) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated (bs-0162R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-iNOS Polyclonal Antibody, Unconjugated(bs-0162R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
中文 
暫時沒有說明書
