產品細節圖:

genefist，GF6619-10，三色預染蛋白marker 10-245KD (protein marker)

品牌：genefist | 貨號：GF6619-10

所屬大類 : 試劑

所屬小類 : 蛋白質/抗原/多肽/酶

品牌 : genefist

規格 : 250ul*10

原價 : ￥2650.00

促銷價 : ￥2650.00

Product Information GeneFist ?series

3-color High Range Protein Marker

(9-245 kDa)

GF6619

Size:

250 μl × 1

5 μl per well for 100 applications

Storage

4°C for 3 months

-20°C for 24 months

Loading Volume

? 3 μl or 5 μl per loading for clear visualization

during electrophoresis on a 15-well or 10-well

mini-gel.

? 1.5~2.5 μl per well for general Western blotting

transfer.

? Apply more for a thicker (> 1.5 mm) or larger gel.Description

The GF6619 GeneFist? 3-color High Range Protein

Marker is a ready to use three-color protein standard

with 12 pre-stained proteins covering a wide range of

molecular weights from 10 to 245 kDa in Tris-Glycine

buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10-

235 kDa in Bis-Tris (MES) buffer). Proteins are

covalently coupled with different chromophores for

easy identification of bands, with two reference

proteins carrying enhanced intensity corresponding

to a green at 25 kDa and red at 75 kDa, respectively,

as separated on SDS-PAGE (Tris-Glycine buffer).

The GF6619 GeneFist? 3-color High Range Protein

Marker is designed for monitoring protein separation

during SDS-polyacrylamide gel electrophoresis,

verification of Western transfer efficiency on

membranes (PVDF, nylon, or nitrocellulose) and for

approximating the size of proteins.

The marker is supplied in gel loading buffer and is

ready to use. Do NOT heat, dilute, or add reducing

agents before loading.Contents

Approximately 0.1~0.4 mg/ml of each protein in the

buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 %

SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v)

Glycerol).

Guide for Molecular Weight Estimation

Migration patterns and approximate MWs (kDa) of

GF6619 GeneFist? 3-color High Range Protein

Marker in different electrophoresis conditions are

listed below:

Band

Color

TRIS

GLYCINE

BIS-TRIS

(MOPS)

BIS-TRIS

(MES)

Blue

245

235

Blue

180

170

Blue

140

130

Blue

100

Red

Blue

Green

Blue

103-color High Range Protein Marker

Note. The apparent molecular weight (kDa) of each protein has been

determined by calibration against an unstained protein standard;

supplemental data should be considered for more accurate

adjustments in different electrophoresis conditions.Quality Control

Under suggested conditions, GF6619 GeneFist? 3-

color High Range Protein Marker resolves 12 major

bands in SDS-PAGE (Tris-Glycine, MOPS, and MES

buffer) and after Western blotting transfer to a

nitrocellulose membrane.

Other information

GENEFIST Technology, Inc. claims all warranties with

respect to this document, expressed or implied,

including but not limited to those of merchantability

or fitness for a particular purpose. In no event shall

GENEFIST Technology, Inc. be liable, whether in

contract, tort, warranty, or under any statute or any

other basis for special, incidental, indirect, punitive,

multiple or consequential damages in connection with

or arising from this document, including but not

limited to the use thereof.

Caution: Not intended for human or animal diagnostic or therapeutic

uses.Related Products

GF6150 GeneFist All Blue Regular Range Protein

Marker, 250 μl × 2

GF6160 GeneFist All Blue Regular Range Plus

Protein Marker, 250 μl × 2

GF6170 GeneFist All Blue Broad Range Protein

Marker, 250 μl × 2

GF6240 GeneFist Pink Blue Protein Marker, 250 μl × 2

GF6616 GeneFist 3-color Regular Range Protein

Marker, 250 μl × 2

GF6619 GeneFist 3-color High Range Protein

Marker, 250 μl × 2

GF6261 GeneFist Enhanced 3-color High Range

Protein Marker, 250 μl × 2

GF6270 GeneFist 3-color Broad Range Protein

Marker, 250 μl × 2

GF6280 GeneFist 3-color Extra Range Protein

Marker, 250 μl × 2

GF6500 GeneFist 3-color Pre-stained Protein Ladder,

Regular Range, 250 μl × 2

GF6510 GeneFist 3-color Pre-stained Protein

Ladder, High Range, 250 μl × 2

GF6520 GeneFist 3-color Pre-stained Protein

Ladder, Broad Range, 250 μl × 2GF2100 GeneFist Protein Fluorescent Staining Dye

(Red, 1,000X), 1 ml

GF5110 GeneFist 50 bp DNA Ladder, 500 μl

GF5220 GeneFist 100 bp DNA Ladder II, 500 μl

GF5310 GeneFist 1 KB (0.25-10 kb) DNA Ladder,

500 μl

GF5510 GeneFist Super Range DNA Ladder, (50 bp-

25 kb), 500 μl

GF3010 GeneFist Blue Light LED epi-illuminator,

AC 100-240V, 50/60Hz

GF1100 GeneFist Western Marker I, 250 μl

GeneFist Western Marker I

For research use only

十二烷基磺酸鈉與十二烷基硫酸鈉的區別
實驗室中的化學試劑的防護之十二烷基硫酸鈉
十二烷基硫酸鈉（SDS）的生物應用

暫時沒有說明書

1.《Multifunction Sr, Co and F co-doped microporous coating on titanium of antibacterial, angiogenic and osteogenic activities》
作者：Jianhong Zhou ,Lingzhou Zhao 影響因子：4.259
期刊：《Scientific Reports 6, Article number: 29069 (2016)》 PMID：27353337

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genefist，GF6619-10，三色預染蛋白marker 10-245KD (protein marker)

品牌：genefist | 貨號：GF6619-10

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